本项目首次在生物样品中实现激光发射，可观察到癌细胞及将要发展成癌细胞的细胞核内DNA变化，提供功能性信息，填补了目前在生命科学研究和疾病诊断中采用的以形态学为主导的空白。与荧光显微镜相比，激光发射显微镜具有窄线宽，阈值行为和高信噪比等优势，从而实现超灵敏检测，出色的图像对比度和更高的光谱/空间分辨率。

       •信噪比 >  50

       •发射光谱 < 0.1nm, 信号集中, 可实现多重激光，同时检测多个靶点

       •与现有临床操作流程匹配，速度更快     

       •图像无需后处理，诊断客观、准确

       用于肿瘤临床病理诊断，潜在可用于术中快速切缘判断、确诊存疑病例、诊断交界性病变、区分亚临床型的肿瘤细胞（即细胞核已经发生肿瘤变异，而形态学尚未形成癌变的细胞）、提示癌变风险等。

       This project is among the first equipment to achieve laser emission in biological samples. It can observe DNA amount or structure changes in the nucleus of cancer and pre-cancerous cells, providing functional evidence beyond morphological changes in pathology samples in basic research and clinical diagnosis. Laser emission has unique advantages over fluorescence, including narrow linewidth, threshold behavior, and strong intensity, leading to ultrasensitive detection, superior image contrast, and higher spectral/spatial resolution.

       •Signal-to-noise ratio > 50

       •Emission bandwidth < 0.1 nm, intense signal and easy to multiplex

       •Similar procedures as currently used in clinics but significantly less time

       •No post-processing needed, objective and accurate

       When used in clinical pathology, laser emission based microscopy could be used for quick diagnosis of surgical margins during surgery, conformational diagnosis of difficult or borderline cases, identification of pre-cancerous cells and oncology risk prediction.